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Trial registered on ANZCTR
Registration number
ACTRN12623001215617p
Ethics application status
Not yet submitted
Date submitted
7/10/2023
Date registered
27/11/2023
Date last updated
27/11/2023
Date data sharing statement initially provided
27/11/2023
Type of registration
Prospectively registered
Titles & IDs
Public title
Effect of Loading on Breathing Behaviour, Muscle Activation, and Performance During Unstable versus Stable Load Resistance Training
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Scientific title
Effect of Loading on Breathing Behaviour, Muscle Activation, and Performance During Unstable versus Stable Load Resistance Training in healthy males and females
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Secondary ID [1]
310755
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None
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Universal Trial Number (UTN)
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Trial acronym
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Linked study record
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Health condition
Health condition(s) or problem(s) studied:
respiratory function
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physical function
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Condition category
Condition code
Respiratory
328463
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0
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Normal development and function of the respiratory system
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Musculoskeletal
328464
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0
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Normal musculoskeletal and cartilage development and function
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Intervention/exposure
Study type
Interventional
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Description of intervention(s) / exposure
The current study will involve one initial testing session and one experimental session. All sessions will be conducted in an exercise clinic at the University of Sydney Camperdown campus (Susan Wakil Health Building). All sessions will be supervised by a strength and conditioning specialist or exercise scientist with one-on-one supervision provided to participants. The interval between the two sessions will be at least 72 hours to avoid the influence of fatigue. A session attendance checklist will be used to monitor adherence to the sessions.
The initial testing session (120 minutes) will encompass in-person screening, anthropometry, blood pressure, spirometry profile, static balance, dynamic balance, core endurance test, core strength test, and the 1RM parallel back squat.
The experimental session (150 minutes) will involve unstable load training (ULT) and traditional training (TRAD) in parallel back squats. The ULT will be the interventional condition, and the TRAD will be the control condition. A recovery period of approximately 30 minutes will be allowed between conditions to minimise the influence of fatigue.
The ULT (interventional condition) will involve a bout of parallel back squats at various loads in ascending order (40% 1RM, 50% 1RM, 60% 1RM, 70% 1RM, 80% 1RM, 90% 1RM) with unstable loads. The unstable load will be achieved by suspending weight plates on both ends of the barbell using elastic bands (Rogue Fitness, Columbus, OH, USA). The bands will be “quadruple looped” through the hole in the centre of each weight plate and hanging on the barbell. Two repetitions at each percentage of 1RM will be performed with a 60-second rest interval between attempts up to 60% 1RM and 120 seconds for 70-90%. The repetition speed of the squats will involve a 3-second eccentric phase followed by maximum velocity/effort in the concentric phase. A 20-kilogram standard Olympic barbell will be utilised for male participants, whilst a 15-kilogram standard Olympic barbell will be utilised for female participants.
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Intervention code [1]
327363
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Treatment: Other
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Comparator / control treatment
The TRAD (control condition) will involve a bout of parallel back squats at various loads in ascending order (40% 1RM, 50% 1RM, 60% 1RM, 70% 1RM, 80% 1RM, 90% 1RM) with stable loads. The stable load will be achieved by placing the plates on both ends of the barbell normally. Two repetitions at each percentage of 1RM will be performed with a 60-second rest interval between attempts up to 60% 1RM and 120 seconds for 70-90%. The repetition speed of the squats will involve a 3-second eccentric phase followed by maximum velocity/effort in the concentric phase. A 20-kilogram standard Olympic barbell will be utilised for male participants, whilst a 15-kilogram standard Olympic barbell will be utilised for female participants.
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Control group
Active
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Outcomes
Primary outcome [1]
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Breath behaviour, including inspiratory volume (Vin), expiratory volume (Vex), inspiratory volume / tidal volume (Vin/Vt), expiratory volume / tidal volume (Vex/Vt), rate of breaths, breath-holding occurrence, and breath-holding duration of each squat, will be collected throughout resistance exercise. All components will be assessed together as a composite outcome.
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Assessment method [1]
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pneumotachograph
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Timepoint [1]
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Monitored continuously throughout resistance exercise.
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Secondary outcome [1]
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Muscle activation of rectus femoris, vastus lateralis, vastus medialis, biceps femoris, soleus, tibialis anterior, rectus abdominis, external oblique, internal oblique, and erector spinae muscles will be monitored throughout resistance exercise. All components will be assessed together as a composite outcome.
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Assessment method [1]
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Surface electromyography (EMG)
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Timepoint [1]
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Monitored continuously throughout resistance exercise.
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Secondary outcome [2]
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Ground reaction force
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Assessment method [2]
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Force platform
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Timepoint [2]
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Monitored continuously throughout resistance exercise.
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Secondary outcome [3]
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Centre of gravity
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Assessment method [3]
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Force platform
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Timepoint [3]
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Monitored continuously throughout resistance exercise.
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Secondary outcome [4]
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Average and maximal velocity
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Assessment method [4]
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Force platformGymAware PowerTool
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Timepoint [4]
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Monitored continuously throughout resistance exercise.
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Secondary outcome [5]
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perceptual fatigue
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Assessment method [5]
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Visual Analog Fatigue Scale (VAFS)
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Timepoint [5]
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Immediately after the exercise session.
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Secondary outcome [6]
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Perceived exertion
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Assessment method [6]
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Rate of perceived exertion (RPE)
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Timepoint [6]
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Immediately after squats in each intensity.
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Eligibility
Key inclusion criteria
1) familiar with back squat; 2) more than 1-year resistance training experience; 3) averaging at least 2 sessions of resistance training per week during the previous 6 weeks.
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Minimum age
18
Years
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Maximum age
35
Years
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Sex
Both males and females
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Can healthy volunteers participate?
Yes
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Key exclusion criteria
1) respiratory system disease; 2) cardiovascular disease; 3) kidney disease; 4) glaucoma; 5) inguinal, femoral, or umbilical hernia; 6) lower back pain; 7) ankle instability; 8) flat feet; and 9) neuromuscular disorders or injuries within the past six months.
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Study design
Purpose of the study
Treatment
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Allocation to intervention
Randomised controlled trial
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Procedure for enrolling a subject and allocating the treatment (allocation concealment procedures)
sealed opaque envelopes
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Methods used to generate the sequence in which subjects will be randomised (sequence generation)
The sequence of the experimental protocols will be randomised by block randomisation (random.org).
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Masking / blinding
Open (masking not used)
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Who is / are masked / blinded?
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Intervention assignment
Crossover
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Other design features
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Phase
Not Applicable
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Type of endpoint/s
Efficacy
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Statistical methods / analysis
Descriptive data will be expressed as mean ±SD. Two-way repeated measures ANOVA will be used to examine the effect of exercise modalities and various intensities on dependent variables. Pearson's correlation coefficients will be calculated. Bonferroni post hoc comparisons will be performed following ANOVA analysis when required. The alpha level will be set at p<0.05.
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Recruitment
Recruitment status
Not yet recruiting
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Date of first participant enrolment
Anticipated
1/03/2024
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Actual
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Date of last participant enrolment
Anticipated
1/09/2024
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Actual
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Date of last data collection
Anticipated
1/10/2024
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Actual
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Sample size
Target
40
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Accrual to date
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Final
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Recruitment in Australia
Recruitment state(s)
NSW
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Funding & Sponsors
Funding source category [1]
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University
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Name [1]
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The University of Sydney
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Address [1]
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Faculty of Medicine & Health, Sydney School of Health Sciences, Discipline of Exercise & Sport Science. Level 6, Western Avenue, D18 - Susan Wakil Health Building, Camperdown, NSW, 2006.
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Country [1]
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Australia
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Primary sponsor type
University
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Name
The University of Sydney
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Address
Faculty of Medicine & Health, Sydney School of Health Sciences, Discipline of Exercise & Sport Science. Level 6, Western Avenue, D18-Susan Wakil Health Building, Camperdown, NSW, 2006.
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Country
Australia
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Secondary sponsor category [1]
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None
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Name [1]
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Address [1]
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Country [1]
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Ethics approval
Ethics application status
Not yet submitted
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Ethics committee name [1]
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The University of Sydney Human Research Ethics Committee (HREC)
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Ethics committee address [1]
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Level 3, Michael Spence Building (F23), Corner of Eastern Avenue and CityRoad, The University of Sydney, Camperdown, NSW, 2006.
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Ethics committee country [1]
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Australia
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Date submitted for ethics approval [1]
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01/01/2024
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Approval date [1]
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Ethics approval number [1]
313959
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Summary
Brief summary
The primary goal of this acute study is to compare breathing behaviour across various loads during parallel back squats between unstable load training (ULT) and traditional training (TRAD). Secondary objectives involve investigating the effects of stable versus unstable conditions across various loads on muscle activation, exercise stability, mechanical performance and perceptual fatigue. The primary hypothesis is that there will be a greater inspiratory volume, a higher rate of breaths, a longer duration of breath-holding, and a larger expiratory volume with an increase in load during squats in both the ULT and TRAD conditions.
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Trial website
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Trial related presentations / publications
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Public notes
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Contacts
Principal investigator
Name
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Dr Daniel Hackett
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Address
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The University of Sydney. Faculty of Medicine & Health, Sydney School of Health Sciences, Discipline of Exercise & Sport Science. Level 6, Western Avenue, D18-Susan Wakil Health Building, Camperdown, NSW, 2006.
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Country
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Australia
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Phone
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+61 424133727
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Fax
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Email
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[email protected]
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Contact person for public queries
Name
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Daniel Hackett
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Address
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The University of Sydney. Faculty of Medicine & Health, Sydney School of Health Sciences, Discipline of Exercise & Sport Science. Level 6, Western Avenue, D18-Susan Wakil Health Building, Camperdown, NSW, 2006.
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Country
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Australia
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Phone
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+61 424133727
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Fax
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Email
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[email protected]
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Contact person for scientific queries
Name
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Daniel Hackett
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Address
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The University of Sydney. Faculty of Medicine & Health, Sydney School of Health Sciences, Discipline of Exercise & Sport Science. Level 6, Western Avenue, D18-Susan Wakil Health Building, Camperdown, NSW, 2006.
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Country
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Australia
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Phone
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+61 424133727
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Fax
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Email
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[email protected]
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Data sharing statement
Will individual participant data (IPD) for this trial be available (including data dictionaries)?
Yes
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What data in particular will be shared?
All of the individual participant data collected during the trial, after de-identification.
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When will data be available (start and end dates)?
Available for 15 years after publication (Starting 1st December 2024 and ending on 30th November 2039).
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Available to whom?
Case-by-case basis at the discretion of the Primary Sponsor.
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Available for what types of analyses?
only to achieve the aims in the approved proposal
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How or where can data be obtained?
Access is subject to approvals by Principal Investigator (Dr Daniel Hackett)
[email protected]
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What supporting documents are/will be available?
No Supporting Document Provided
Doc. No.
Type
Citation
Link
Email
Other Details
Attachment
20626
Ethical approval
Once ethics approval is received.
Results publications and other study-related documents
Documents added manually
No documents have been uploaded by study researchers.
Documents added automatically
No additional documents have been identified.
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